HLA-A2.1/B2M(M-NSG)(2)

品系全名

NOD.Cg-PrkdcscidIl2rgem1B2mtm1(hB2M-linker-HLA-A*0201/H2-D1)Smoc

目录号

NM-HU-241683

品系状态

精子冻存

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基因信息

基因名
B2M

基因曾用名

y-m11; beta2m; beta2-m

NCBI ID

12010

MGI ID

88127

Ensembl ID

ENSMUSG00000060802

Pubmed

B2M

人类同源基因

B2M

品系描述

利用同源重组，将小鼠B2m基因进行人源化修饰。

验证数据

image.png

Fig.1 Detection of hB2M and HLA-A,B,C expression on leukocytes in blood of WT M-NSG mice and HLA-A2.1/B2M (M-NSG)(2) mice by FACS. 

Leukocytes were collected from wild-type M-NSG mice (male, 6~8 weeks old) and HLA-A2.1/B2M (M-NSG)(2) humanized mice (male, 8 weeks old), and analyzed by flow cytometry with anti-HLA-ABC, anti-mH2D, anti-mB2M, and anti-hB2M.

Abbr. HO, homozygous; WT, wild type.

image.png

Fig.2 Detection of HLA-A expression on leukocytes in blood of WT M-NSG mice and HLA-A2.1/B2M (M-NSG)(2) mice by FACS.

Leukocytes were collected from wild-type M-NSG mice (male, 8 weeks old) and HLA-A2.1/B2M (M-NSG)(2) humanized mice (female, 6 weeks old), and analyzed by flow cytometry with anti-HLA-A and anti-mB2M.

Abbr. HO, homozygous; WT, wild type.

image.png

Fig.3 Detection of HLA-A expression on leukocytes in spleen of WT M-NSG mice and HLA-A2.1/B2M (M-NSG)(2) mice by FACS.

Leukocytes were collected from wild-type M-NSG mice (male, 8 weeks old) and HLA-A2.1/B2M (M-NSG)(2) humanized mice (female, 6 weeks old), and analyzed by flow cytometry with anti-HLA-Aand anti-mB2M.

Abbr. HO, homozygous; WT, wild type.


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