hCD98/5xFAD

通过hCD98(NM-HU-234539)与5xFAD小鼠交配获得。

Fig.1 Detection of CD98 expression in 5xFAD/hCD98 mice by WB. Human CD98 was exclusively detectable in brain from 5xFAD/hCD98 mice. The brain lysates were collected from 13-week-old wild-type C57BL/6 mice and 12-week-old heterozygous/homozygous 5xFAD/hCD98 mice, and then analyzed by western blot with anti-CD98 antibody. 

Abbr. HE, Heterozygous; HO, homozygous; WT, wild type; PC, positive control, Hela cells; M, marker.

Note. The mice did not undergo fasting before collecting tissues.

Table 2. Blood routine examination of 5xFAD/hCD98 mice. Blood routine parameters in 5xFAD/hCD98 knock-in mice were similar to those in wild-type C57BL/6 mice. Values are expressed as mean±SEM. 

Abbr. HE, heterozygous; HO, homozygous; WT, wild type.

Note. The mice did not undergo fasting before collecting the blood samples.

Table 3. Biochemical test of serum from HE/HO 5xFAD/hCD98 knock-in mice. Values are expressed as mean±SEM.

Abbr. HE, heterozygous; HO, homozygous; WT, wild type.

Note. The mice did not undergo fasting before collecting the serum samples.

Fig.2 Biochemical test of serum from HE/HO 5xFAD/hCD98 knock-in mice. Male 5xFAD/hCD98 mice showed no significant differences in serum liver, renal, or lipid parameters or phosphorus levels compared with WT males. Female 5xFAD/hCD98 mice exhibited slightly higher serum ALT, AST, UREA, and phosphorus levels than WT females, but remained comparable to 5xFAD/hCD98 males. (Mean±SEM, Unpaired t-test, *p<0.05, **p<0.01, ns: no significance).

Abbr. HE, heterozygous; HO, homozygous; WT, wild type.

Note. The mice did not undergo fasting before collecting the serum samples.