hCD3EDG/hCDCP1(2)
品系全名
C57BL/6JSmo-Cdcp1tm1(hCDCP1)Cd3etm1(hCD3E)Cd3dtm1(hCD3D)Cd3gtm1(hCD3G)Smoc
目录号
NM-XA-253682
品系状态
活体
品系描述
验证数据
Fig.1 Detection of CDCP1 expression in colon by RT-PCR. Mouse Cdcp1 mRNA (194 bp) was detectable only in wild-type C57BL/6 mice. Human CDCP1 mRNA (206 bp) was detectable only in homozygous hCD3EDG/hCDCP1(2) mice but not in wild-type mice. Colon RNA was extracted from 6-7-week-old male wild-type C57BL/6 (WT)(n=2) and 7-week-old homozygous hCD3EDG/hCDCP1(2) mice (HO/HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Fig.2 Detection of CDCP1 expression in skin, testis, heart, liver, kidney, lung, brain, colon and skeletal muscle by qPCR. Human CDCP1 mRNA was detectable only in homozygous hCD3EDG/hCDCP1(2) mice but not in wild-type mice. Tissues RNA were extracted from 6-7-week-old male wild-type C57BL/6 (WT)(n=2) and 7-week-old homozygous hCD3EDG/hCDCP1(2) mice (HO/HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with human CDCP1 and mouse Gapdh primers. Relative expression represents the human CDCP1 mRNA level relative to its average expression of lung in homozygous hCDCP1(2) knockin mice .
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.3 Detection of CDCP1 expression in skin, testis, heart, liver, kidney, lung, brain, colon and skeletal muscle by qPCR. Mouse Cdcp1 mRNA was detectable only in wild-type mice but not in homozygous hCD3EDG/hCDCP1(2) mice. Tissues RNA were extracted from 6-7-week-old male wild-type C57BL/6 (WT)(n=2) and 7-week-old homozygous hCD3EDG/hCDCP1(2) mice (HO/HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with mouse Cdcp1 and mouse Gapdh primers. Relative expression represents the mouse Cdcp1 mRNA level relative to its average expression of lung in wild-type mice .
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.4 Detection of CDCP1 expression in hCD3EDG/hCDCP1(2) mice by WB. The heart, liver, lung, kidney, brain, skeletal muscle, colon, uterus and skin lysates were collected from 6-week-old female wild-type C57BL/6 mice and 6-week-old female homozygous hCD3EDG/hCDCP1(2) mice, and then analyzed by western blot with anti-CDCP1 antibody.
Abbr. HO, homozygous; WT, wild type; PC, positive control, HCT-116 cells; M, marker.
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