Tnfaip6-KO

Fig.1 Detection of Tnfaip6 expression in bone marrow, spleen and cerebellum by qPCR. 

All mice were administered 5 mg/kg LPS (or saline) by intraperitoneal (i.p.) injection before 2 h of sacrifice. mRNA expression was reported as the percentage to Tnfaip6 mRNA levels in the cerebellum of WT mice (n=2, 10-week-old, female). 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of Tnfaip6 expression in bone marrow, spleen and cerebellum by RT-PCR (10-week-old, female). 

Wild type: one band at 687 bp with primers F1/R2(mTnfaip6) and one band at 123 bp with primers F3/R3(mGapdh).

Homozygous: one band at 158 bp with primers F1/R2(mTnfaip6) and one band at 123 bp with primers F3/R3(mGapdh).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.