hPSMA

品系全名

C57BL/6Smoc-Folh1em1(hPSMA-Wpre-PA)Smoc

目录号

NM-HU-200240

品系状态

活体

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基因信息

基因名
Folh1

品系描述

通过同源重组,将小鼠Folh1基因进行人源化修饰。

验证数据

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Fig1. Detection of PSMA expression in kidney by RT-PCR. Wild type: only one band at 294 bp with primers F1/R1(mPsma);Heterozygous: one band at 294 bp with primers F1/R1(mPsma) and  one band at 169 bp with primers F2/R2(hPSMA); Abbr.. M, DNA marker; HO, homozygous; HE, heterozygous; WT, wild type.

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Fig2. Detection of hPSMA expression in prostate in hPSMA KI mice.

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Fig3. Detection of human PSMA expression in Prostate by IHC.

Note. The human PSMA antibody cross-reacted with mouse PSMA and human PSMA. Abbr. HO, homozygous; HE, heterozygous; WT, wild type.


NM-HU-200240 hPSMA-1.png

Fig4. Analysis of human PSMA expression in the MC38-hPSMA cell line and the transplanted tumor of the PSMA humanized mice by FACS. Most of the MC38-hPSMA cells express human PSMA (A,B);  the transplanted tumor formed by MC38-hPSMA cell line expresses human PSMA on the CD45- cells (C).(Completed in collaboration with CrownBio)

NM-HU-200240 hPSMA-2.png

Fig5. Subcutaneous tumor formation experiment of the MC38-hPSMA cells in the PSMA humanized mice .The tumor volume was measured every 5 days after injection of MC38-hPSMA cells (n=10).The previous studies have shown that wild type mice would spontaneously reject the syngeneic tumors  expressing an introduced human tumor antigen-hPSMA. While the MC38-hPSMA cells can form tumors subcutaneously in the humanized PSMA mice, indicating that the PSMA humanized mouse model can be used to transplant the MC38-hPSMA cell line.(Completed in collaboration with CrownBio)


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