hSIGLEC10
品系全名
C57BL/6JSmo-Siglecgtm1(hSIGLEC10)/Smoc
目录号
NM-HU-2000061
品系状态
胚胎冻存
基因信息
基因名
Siglecg
品系描述
验证数据
图1. SIGLEC10人源化小鼠脾脏中人源SIGLEC10表达的 RT-PCR检测结果。
引物对P1/2特异针对野生型(WT)序列，WT小鼠可扩增出812bp产物，HO（纯合子）无条带；
引物对P3/2特异针对SIGLEC10人源化小鼠序列，HO小鼠可扩增出394bp产物，WT无条带。
M：100bp Plus DNA ladder from Transgen (BM311)
图2. FACS 检测SIGLEC10人源化小鼠外周血及脾脏CD19+细胞中人源SIGLEC10的表达。
结果显示SIGLEC10人源化小鼠外周血及脾脏CD19+细胞中仅有人源SIGLEC10表达，而在野生型小鼠中仅有鼠源Siglec-G表达。
anti-mouse Siglec-G：BD Pharmingen™,563336；
anti-human Siglec-10：Biolegend，347603.
图3. SIGLEC10人源化小鼠体内药效研究结果示例。结果显示，CD24 mAb1和CD24 mAb2可抑制MC38-hCD24移植瘤的生长（A），且对小鼠体重无明显影响（B）。
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