hPD-L2
品系全名
C57BL/6JSmo-Pdcd1lg2tm1(hPDCD1LG2)Smoc
目录号
NM-HU-190050
品系状态
胚胎冻存
基因信息
基因名
Pdcd1lg2
品系描述
验证数据
图1. PD-L2人源化小鼠构建策略。
图2. RT-PCR鉴定结果图。引物对 P1/2 特异针对野生型小鼠，引物对 P3/4 特异针对PD-L2人源化小鼠。WT小鼠用 P1/2 引物对可扩增出 404bp 产物;人源化小鼠用 P3/4 引物对可扩增出 405 bp 产物。
引物对 P1/2 Wild type = 404 bp
引物对 P3/4 mutant = 405 bp
M:100bp Plus DNA ladder from Transgen (BM311)
图3 FACS 检测PD-L2人源化小鼠脾脏中人源PD-L2的表达。
PD-L2人源化小鼠通过尾静脉注射刀豆蛋白（12mg/kg）刺激4h，取脾脏细胞进行流式验证。结果显示： PD-L2人源化杂合子小鼠脾脏中既可以检测到人源PD-L2的表达，也可以检测到鼠源PD-L2 的表达；而PD-L2人源化纯合子小鼠脾脏中只检测到人源PD-L2的表达。
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