hIL4RA

品系全名

C57BL/6JSmo-Il4ratm1(hIL4R)Smoc

目录号

NM-HU-190008

品系状态

胚胎冻存

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基因信息

基因名
IL4Ra

品系描述

通过同源重组,将小鼠Il4ra基因进行人源化修饰。
应用领域:免疫治疗;肿瘤研究;药物筛选

验证数据

mRNA表达RT-PCR鉴定结果

IL4R-2.png

图1 鉴定结果图。引物对P1/2特异针对鼠源序列,引物对P3/4特异针对人源序列。HE(杂合子)和WT(野生型)小鼠用P1/2引物对可扩增出487bp产物。HE小鼠用P3/4引物对可扩增出372bp产物,WT无条带。M:100bp Plus DNA ladder.

HuIL4RA表达量检测结果

HuIL4RA.png

图2. IL4RA-HU小鼠HuIL4RA表达量的检测。 IL4RA-HU小鼠内眦静脉取血,分离血清,稀释10倍后进行Elisa检测。(HO: homozygote ; WT:Wild type ; ND:Not detected)

MuIL4RA表达量检测结果

MuIL4RA.png

图3. IL4RA-HU小鼠MuIL4RA表达量的检测。 IL4RA-HU小鼠内眦静脉取血,分离血清,稀释10倍后进行Elisa检测。(HO: homozygote ; WT:Wild type ; ND:Not detected)


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