Cdkn2a-Luc-2A-tdTomato-2A-CreERT2(p16-Luc-2A-tdTomato-2A-CreERT2)

品系全名

C57BL/6JSmo-Cdkn2aem(Luc-tdTomato-CreERT2)Smoc

目录号

NM-KI-18039

品系状态

活体

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基因信息

基因名
Cdkn2a

基因曾用名

Arf; p16; MTS1; Pctr1; p19ARF; p16INK4a; p19; ARF-INK4a; INK4a-ARF; Ink4a/Arf; p16(INK4a)

NCBI ID

12578

MGI ID

104738

Ensembl ID

ENSMUSG00000044303

Pubmed

Cdkn2a

人类同源基因

CDKN2A

品系描述

将Luc-2A-tdTomato-2A-CreERT2-WPRE-pA定点插入到p16INK4a转录本exon 2中，该表达结构位于第62个氨基酸之后。

验证数据


Cdkn2a-2.png

图1 MEF细胞Luciferase荧光值检测。结果Cdkn2a KI的细胞有Luciferase的表达。

检测步骤：

1、细胞裂解检后检测Luciferase荧光值；

2、Bradford法检测每个样本的蛋白浓度；

3、荧光值/蛋白量作为统计值。

发表文献 6篇

1. Intermittent hypobaric pressure induces selective senescent cell death and alleviates age-related osteoporosis
发表年份：2026 来源杂志：Nature Biomedical Engineering

2. Reversible ALKBH5 cytosolic aggregation accelerates cellular senescence
发表年份：2025 来源杂志：CELL DEATH AND DIFFERENTIATION

3. Senescent-like macrophages mediate angiogenesis for endplate sclerosis via IL-10 secretion in male mice
发表年份：2024 来源杂志：Nature Communications

4. Radiotherapy-Induced Astrocyte Senescence Promotes an Immunosuppressive Microenvironment in Glioblastoma to Facilitate Tumor Regrowth
发表年份：2024 来源杂志：Advanced Science

5. Apoptotic metabolites ameliorate bone aging phenotypes via TCOF1/FLVCR1-mediated mitochondrial homeostasis
发表年份：2024 来源杂志：JOURNAL OF NANOBIOTECHNOLOGY

6. Senescent-like macrophages mediateangiogenesis for endplate sclerosis via IL-10secretioninmalemice
发表年份：2024 来源杂志：Nature Communications

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