hCD3EDG/hBCMA/hGPRC5D
品系全名
C57BL/6JSmo-Tnfrsf17tm1(hBCMA)Cd3etm1(hCD3E)Cd3dtm1(hCD3D)Cd3gtm1(hCD3G)Gprc5dtm1(hGPRC5D)Smoc
目录号
NM-XA-242725
品系状态
活体
基因信息
基因名
Adora2a
品系描述
验证数据
Fig.1 Detection of GPRC5D expression in hCD3EDG/hBCMA/hGPRC5D mice by WB.
The colon, spleen and kidney tissue lysates were collected from 6-week-old male/female wild-type C57BL/6 mice and 7-week-old male/female homozygous hCD3EDG/hBCMA/hGPRC5D mice, and then analyzed by western blot with anti-GPRC5D antibody.
Abbr. HO, homozygous; WT, wild type; M, marker.
Fig 2. Frequency of leukocyte subpopulations in the blood by flow cytometry
Blood was collected from WT C57BL/6 mice and HO hCD3EDG/hBCMA/hGPRC5D mice (female,7-week-old, n=3). Flow cytometry analysis of frequencies of lymphocyte subpopulations in the blood. Bar indicated expressed as mean ± SEM.
Abbr. HO, homozygous; WT, wild type.
Fig 3. Frequency of leukocyte subpopulations in the blood by flow cytometry
Blood was collected from WT C57BL/6 mice and HO hCD3EDG/hBCMA/hGPRC5D mice (male,7-week-old, n=3). Flow cytometry analysis of frequencies of lymphocyte subpopulations in the blood. Bar indicated expressed as mean ± SEM.
Abbr. HO, homozygous; WT, wild type.
Fig 4. Frequency of leukocyte and myeloid subpopulations in the spleen by flow cytometry
Spleen was collected from WT C57BL/6 mice and HO hCD3EDG/hBCMA/hGPRC5D mice (female,7-week-old, n=3). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the blood. Bar indicated expressed as mean ± SEM.
Abbr. HO, homozygous; WT, wild type.
Fig 5. Frequency of leukocyte and myeloid subpopulations in the spleen by flow cytometry
Spleen was collected from WT C57BL/6 mice and HO hCD3EDG/hBCMA/hGPRC5D mice (male,7-week-old, n=3). Flow cytometry analysis of frequencies of (A) lymphocyte and (B) myeloid subpopulations in the blood. Bar indicated expressed as mean ± SEM.
Abbr. HO, homozygous; WT, wild type.
Fig 6. Frequency of leukocyte subpopulations in the LN by flow cytometry
LN was collected from WT C57BL/6 mice and HO hCD3EDG/hBCMA/hGPRC5D mice (female,7-week-old, n=2). Flow cytometry analysis of frequencies of lymphocyte subpopulations in the blood. Bar indicated expressed as mean ± SEM.
Abbr. HO, homozygous; WT, wild type.
Fig 7. Frequency of leukocyte subpopulations in the LN by flow cytometry
LN was collected from WT C57BL/6 mice and HO hCD3EDG/hBCMA/hGPRC5D mice (male,7-week-old, n=3). Flow cytometry analysis of frequencies of lymphocyte subpopulations in the blood. Bar indicated expressed as mean ± SEM.
Abbr. HO, homozygous; WT, wild type.
Table 1. Blood routine examination of HO hCD3EDG/hBCMA/hGPRC5D mice. The baseline physiological parameters of HO hCD3EDG/hBCMA/hGPRC5D mice showed no significant abnormalities.
Abbr. HO, homozygous; WT, wild type. Values are expressed as mean±SD.
Table 2. Biochemical test of serum from HO hCD3EDG/hBCMA/hGPRC5D mice.
Abbr. HO, homozygous; WT, wild type. Values are expressed as mean±SD.
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