hTACR2

Fig.1 Detection of TACR2 expression in adrenal by RT-PCR. Mouse Tacr2 mRNA (163 bp) was detectable only in wild-type C57BL/6 mice. Human TACR2 mRNA (221 bp) was detectable only in homozygous hTACR2 knockin mice but not in wild-type mice. Adrenal RNA was extracted from 6-week-old male wild-type C57BL/6 (WT)(n=2) and homozygous hTACR2 knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of TACR2 expression in hTACR2 mice by WB. TACR2 was detectable in colon and trachea from both WT C57BL/6 and HO hTACR2 mice , as the antibody was cross-reactived between human and mouse TACR2. The colon and trachea tissue lysates were collected from 6-week-old male wild-type C57BL/6 mice and homozygous hTACR2 mice, and then analyzed by western blot. 

Abbr. HO, homozygous; WT, wild type; PC, positive control, Hela cells.

Fig.3 Detection of mouse neurokinin A (NKA) levels in small intestine homogenates of WT C57BL/6 mice and hTACR2 knockin mice by ELISA. The expression levels of mouse Neurokinin A (NKA) in small intestine homogenates were similar between WT C57BL/6 and homozygous hTACR2 knockin mice. Small intestine homogenate were collected from wild-type C57BL/6 mice (n=2, male, 6 weeks old) and HO hTACR2 mice (n=2, male, 6 weeks old), and analyzed by ELISA.

Abbr.  HO, homozygous; WT, wild type.