hAREG

Fig.1 Detection of AREG expression in mammary gland by RT-PCR. Mouse Areg mRNA (331bp) was detectable only in wild-type C57BL/6 mice. Human AREG mRNA (330 bp) was detectable only in homozygous hAREG knockin mice but not in wild-type mice. Mammary gland RNA was extracted from 6-week-old male wild-type C57BL/6 (WT)(n=2), 6-week-old male homozygous hAREG knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of AREG expression in hAREG mice by WB. AREG was detectable in testis, lung, stomach and brain from both WT C57BL/6 and HO hAREG mice , as the antibody was cross-reactived between human and mouse AREG. The testis, lung, stomach and brain tissue lysates were collected from 6-week-old male wild-type C57BL/6 mice and 6-week-old male homozygous hAREG mice, and then analyzed by western blot with anti-AREG antibody. 

Abbr. HO, homozygous; WT, wild type; PC, positive control, Hela cells.