HLA-A11.1/B2M(2)

Fig.1 Expression analysis of hB2M and HLA-ABC on CD45+ cells in blood of HLA-A11.1/B2M humanized mice. 

Leukocytes were collected from wild-type C57BL/6 mice(11 weeks old)  and homozygous HLA-A11.1/B2M humanized mice(15.7 weeks old) , and analyzed by flow cytometry with anti-mB2M, anti-hB2M, anti-mH2D and anti-HLA-ABC. hB2M and HLA-ABC were exclusively detectable in HLA-A11.1/B2M humanized mice, whereas mB2M and mH2D were exclusively detectable in wild-type C57BL/6 mice.

Fig.2 Analysis of immune cell subpopulations in the blood (n=3 per group). 

Leukocytes were collected from the blood of wild-type C57BL/6 mice (female, 8 weeks old; male, 9 weeks) and homozygous HLA-A11.1/B2M humanized mice (9 weeks old), and Flow cytometry analysis was performed to assess leukocyte subpopulations.

Abbr.  WT, wild type; HO, homozygous.

Fig.3 Analysis of immune cell subpopulations in the spleen (n=3 per group). 

Leukocytes were collected from the spleens of wild-type C57BL/6 mice (female, 8 weeks old; male, 9 weeks) and homozygous HLA-A11.1/B2M humanized mice (9 weeks old), and flow cytometry analysis was performed to assess leukocyte subpopulations.

Abbr.  WT, wild type; HO, homozygous.