hF2RL1

Fig.1 Detection of F2RL1 expression in pancreas, stomach, ileum, large intestine and duodenum by RT-PCR. 

Wild type: only one band at 169 bp with primers F1/R1(mF2rl1);

Homozygous: only one band at 162 bp with primers F2/R2(hF2RL1).

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

Fig.2 Detection of hF2RL1 (A) and mF2RL1 (B) expression in pancreas, stomach, ileum, large intestine and duodenum by qPCR. (female, 8 weeks old)

(A) Relative expression represents the hF2RL1 mRNA levels relative to its average expression in the stomach of hF2RL1 knockin mice. 

(B) Relative expression represents the mF2rl1 mRNA levels relative to its average expression in the stomach of WT mice. 

Abbr. HO, homozygous; WT, wild type.

Fig.3 Detection of human F2RL1 expression in hF2RL1 knockin mice by WB. 

Abbr. M, marker; WT, wild type; HO, Homozygous; PC, positive control, HepG2 cells.