hITGB7

Fig.1 Detection of ITGB7 expression in spleen by RT-PCR. Spleen RNA was extracted from 8-10-week-old male WT C57BL/6 mice and 9-week-old male homozygous hITGB7 knockin mice, then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse Itgb7 mRNA (274 bp) was detectable only in wild-type C57BL/6 mice. Human ITGB7 mRNA (536 bp) was detectable only in homozygous hITGB7 knockin mice but not in wild-type mice. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of human ITGB7 expression on T cells and B cells in blood of WT mice and hITGB7 knockin mice by FACS. Blood leukocytes were collected from a WT C57BL/6 mouse (male, 11 weeks old) and homozygous hITGB7 knockin mice (male, 11 weeks old), and analyzed by flow cytometry with an anti-hITGB7 antibody. Human ITGB7 was exclusively detectable on T cells and B cells in blood of hITGB7 knockin mice but not in WT mice. 

Abbr. HO, homozygous; WT, wild type.

Fig.3 Detection of human ITGB7 expression on T cells and B cells in spleen of WT mice and hITGB7 knockin mice by FACS. Splenic leukocytes were collected from a WT C57BL/6 mouse (male, 11 weeks old) and homozygous hITGB7 knokcin mice (male, 11 weeks old), and analyzed by flow cytometry with anti-hITGB7. The expression ratios of LPAM-1 (α4β7 heterodimer) on blood T and B cells were comparable between WT C57BL/6 and homozygous hITGB7 knockin mice.

Abbr. HO, homozygous; WT, wild type.

Fig.4 Detection of LPAM-1 expression on T cells and B cells in blood of WT mice and hITGB7 knockin mice by FACS. Blood leukocytes were collected from a WT C57BL/6 mouse (male, 11 weeks old) and homozygous hITGB7 knockin mice (male, 11 weeks old), and analyzed by flow cytometry with an anti-mLPAM-1 antibody. The expression ratios of LPAM-1 (α4β7 heterodimer) on blood T and B cells were comparable between WT C57BL/6 and homozygous hITGB7 knockin mice.

Abbr. HO, homozygous; WT, wild type.

Fig.5 Detection of LPAM-1 expression on T cells and B cells in spleen of WT mice and hITGB7 knockin mice by FACS. Splenic leukocytes were collected from a WT C57BL/6 mouse (male, 11 weeks old) and homozygous hITGB7 knockin mice (male, 11 weeks old), and analyzed by flow cytometry with an anti-mLPAM-1 antibody. The expression ratios of LPAM-1 (α4β7 heterodimer) on spleen T and B cells were comparable between WT C57BL/6 and homozygous hITGB7 knockin mice.

Abbr. HO, homozygous; WT, wild type.