hAPP(3)

Fig.1 Detection of APP expression in cerebellum, cortex, frontal lobe and lung by RT-PCR. Cerebellum, cortex, frontal lobe and lung RNA were extracted from 6-week-old male wild-type C57BL/6 (WT)(n=3) and heterozygous hAPP(3) knockin mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse App mRNA (319 bp) was detectable in both wild-type C57BL/6 mice and heterozygous hAPP(3) knockin mice. Human APP mRNA (282 bp) was detectable only in heterozygous hAPP(3) knockin mice but not in wild-type mice.

Abbr. M, marker; HE, heterozygous; WT, wild type.

Fig.2 Detection of APP expression in cerebellum, cortex, frontal lobe and lung by RT-PCR. Mouse App mRNA (319 bp) was detectable only in wild-type C57BL/6 mice. Human APP mRNA (282 bp) was detectable only in heterozygous hAPP(3) knockin mice but not in wild-type mice. Cerebellum, cortex, frontal lobe and lung RNA were extracted from 5-week-old male wild-type C57BL/6 (WT)(n=3) and homozygous hAPP(3) knockin mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.3 Detection of APP expression in hAPP(3) mice by WB. The hippocampus, cortex, liver and cerebellum tissue lysates were collected from 6-week-old male wild-type C57BL/6 mice and 6-week-old male heterozygous hAPP(3) mice, and then analyzed by western blot with anti-APP antibody. APP was detectable in hippocampus, cortex, liver and cerebellum from both WT C57BL/6 and HE hAPP(3) mice , as the antibody was cross-reactived between human and mouse APP. 

Abbr. HE, heterozygous; WT, wild type; PC, positive control, Hela cells.

Fig.4 Detection of APP expression in hAPP(3) mice by WB. APP was detectable obviously in cortex, hippocampus and cerebellum while not obvious in liver from HO hAPP(3) mice with a human APP-specific antibody. The cortex, hippocampus, cerebellum and liver tissue lysates were collected from 6-week-old male wild-type C57BL/6 mice and 6-week-old male homozygous hAPP(3) mice, and then analyzed by western blot. 

Abbr. HO, homozygous; WT, wild type; PC, positive control, Hela cells.