hPCSK9(4)

Fig.1 Detection of PCSK9 expression in liver by RT-PCR. Human PCSK9 mRNA (138 bp) was detectable only in homozygous hPCSK9(4) knockin mice but not in wild-type mice. Liver RNA was extracted from 7-week-old male wild-type C57BL/6 (WT)(n=3) and homozygous hPCSK9(4) knockin mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers. Mouse Pcsk9 mRNA (154 bp) was detectable only in wild-type C57BL/6 mice. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of human PCSK9 (A) expression in serum of WT C57BL/6 mice and HE hPCSK9(4) mice by ELISA.  Human PCSK9 was exclusively detectable in heterozygous hPCSK9(4) knockin mice. Serum were collected from wild-type C57BL/6 mice (n=2, male, 12 weeks old) and HE hPCSK9(4) mice (n=3, male, 14 weeks old), and analyzed by ELISA. 

Abbr.  HE, heterozygous; WT, wild type.

Fig.3 Detection of human PCSK9 (A) expression in serum of WT C57BL/6 mice and HO hPCSK9(4) mice by ELISA.  Human PCSK9 was exclusively detectable in heterozygous hPCSK9(4) knockin mice. Serum were collected from wild-type C57BL/6 mice (n=3, male, 6 weeks old) and HO hPCSK9(4) mice (n=3, male, 6 weeks old), and analyzed by ELISA. 

Abbr.  HO, homozygous; WT, wild type.