hFGFR1/hGCGR/hGLP1R

Fig.1 Detection of FGFR1 expression in hFGFR1/hGCGR/hGLP1R mice by WB. FGFR1 was detectable in heart, liver, lung, kidney and gastrocnemius muscle from both WT C57BL/6 and HO/HO/HO hFGFR1/hGCGR/hGLP1R mice , as the antibody was cross-reactive between human and mouse FGFR1. The heart, liver, lung, kidney and gastrocnemius muscle tissue lysates were collected from 10-week-old male wild-type C57BL/6 mice and 10-week-old male homozygous hFGFR1/hGCGR/hGLP1R mice, and then analyzed by western blot with anti-FGFR1 antibody. 

Abbr. HO, homozygous; WT, wild type; PC, positive control, HepG2 cells.

Fig.2 Detection of GCGR expression in hFGFR1/hGCGR/hGLP1R mice by WB. GCGR was detectable in liver and kidney from both WT C57BL/6 and HO/HO/HO hFGFR1/hGCGR/hGLP1R mice, as the antibody was cross-reactive between human and mouse GCGR. The liver and kidney tissue lysates were collected from 10-week-old male wild-type C57BL/6 mice and 10-week-old male homozygous hFGFR1/hGCGR/hGLP1R mice, and then analyzed by western blot with anti-GCGR antibody. 

Abbr. HO, homozygous; WT, wild type; PC, positive control, HepG2 cells.

Fig.3 Detection of human GLP-1R expression in hFGFR1/hGCGR/hGLP1R mice by IHC. Human GLP-1R was exclusively detectable in pancreatic islets and neurons located in the hypothalamus and medulla from HO/HO/HO hFGFR1/hGCGR/hGLP1R mice but not in WT mice. Tissues were collected from 10-week-old male WT C57BL/6 mice and 10-week-old male HO/HO/HO hFGFR1/hGCGR/hGLP1R mice, then analyzed by IHC with anti-GLP-1R antibody. Black arrows indicate positive staining (brown). Scale bar: 100 μm. Magnification, 200×.

Abbr. HO, homozygous; WT, wild type.

Fig.4 Detection of human GLP-1R expression in hFGFR1/hGCGR/hGLP1R mice by IHC. Human GLP-1R was exclusively detectable in the kidney, stomach, and the myenteric plexus of the ileum from HO/HO/HO hFGFR1/hGCGR/hGLP1R mice but not in WT mice. Tissues were collected from 10-week-old male WT C57BL/6 mice and 10-week-old male HO/HO/HO hFGFR1/hGCGR/hGLP1R mice, then analyzed by IHC with anti-GLP-1R antibody. Black arrows indicate positive staining (brown). Scale bar: 100 μm. Magnification, 200×.

Abbr. HO, homozygous; WT, wild type.

Table 1. Biochemical test of serum from HO/HO/HO hFGFR1/hGCGR/hGLP1R knockin mice without fasting. Values are expressed as mean±SD.

Abbr. HO, homozygous; WT, wild type.

Table 2. Biochemical test of serum from fasted HO/HO/HO hFGFR1/hGCGR/hGLP1R knockin mice. Values are expressed as mean±SD.

Abbr. HO, homozygous; WT, wild type.