hCB1(2)

利用同源重组，将小鼠Cnr1基因进行人源化修饰。

Fig.1 Detection of CNR1 expression in cerebellum by RT-PCR. Cerebellum RNA was extracted from wild-type C57BL/6 mice and homozygous hCB1(2) knockin mice, then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human mRNA primers (n=2, male, 7 weeks old). Mouse Cnr1 mRNA (373 bp) was detectable only in wild-type C57BL/6 mice. Human CNR1 mRNA (209 bp) was detectable only in homozygous hCB1(2) knockin mice but not in wild-type mice. 

Abbr. M, marker; HO, homozygous; WT, wild type.

Fig.2 Detection of CB1 expression in hCB1(2) mice by WB. 

The brain, liver, kidney, mammary gland and BAT tissue lysates were collected from female 7-week-old wild-type C57BL/6 mice and homozygous hCB1(2) mice, and then analyzed by western blot with anti-CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. M, marker; BAT, brown adipocyte tissue.

Note. The arrow indicates the target strip.