hTNFR2(BALB/c)

Fig.1 Expression analysis of hTNFR2 on Treg cells in spleen of hTNFR2 humanized mice. 

Leukocytes were collected from spleen of wild-type balb/c mice (female, 8 weeks) and homozygous hTNFR2 mice (male, 14 weeks) stimulated in vivo with anti-CD3ε (50 µg/mouse, 24-hour stimulation, i.v.) and analyzed by flow cytometry with anti-mTNFR2 and anti-hTNFR2. hTNFR2 were exclusively detectable in hTNFR2 humanized mice but not in wild-type mice.

Fig.2 Expression analysis of hTNFR2 on DCs, monocyte, B cells, and neutrophils in spleen of hTNFR2 humanized mice. 

Leukocytes were collected from spleen of wild-type balb/c mice (female, 12 weeks) and homozygous hTNFR2 mice (male, 18 weeks), and analyzed by flow cytometry with anti-mTNFR2 and anti-hTNFR2. hTNFR2 were exclusively detectable in hTNFR2 humanized mice but not in wild-type mice.

Fig.3 Expression analysis of hTNFR2 on monocyte, B cells, and neutrophils in blood of hTNFR2 humanized mice. 

Leukocytes were collected from blood of wild-type balb/c mice (female, 12 weeks) and homozygous hTNFR2 mice (male, 18 weeks), and analyzed by flow cytometry with anti-mTNFR2 and anti-hTNFR2. hTNFR2 were exclusively detectable in hTNFR2 humanized mice but not in wild-type mice.