R26-CAG-LSL-hPLA2R1-IRES-EGFP

Fig.1 Mice overexpressing hPLA2R1 exhibited slower body weight gain and increased mortality (n=12~20/group).

Fig.2 Urinary biochemical results. 

hPLA2R1-overexpressing mice exhibited urinary biochemical abnormalities starting at 4 weeks of age (n=7/group).

Fig.3 Blood biochemical results. 

hPLA2R1-overexpressing mice exhibited blood biochemical abnormalities starting at 4 or 6 weeks of age. (n=7/group).

Fig.4 Immunofluorescence staining of hPLA2R1 in the kidney with Anti-PLA2R antibody (Abcam, ab211573).R26^{hPLA2R1-EGFP/hPLA2R1-EGFP}; NPHS2-Cre+ mice showed sustained PLA2R1 expression.

Fig.5 Immunofluorescence staining of mIgG (green) and lamin A/C (red) in the kidney. 

Overall, hPLA2R1-overexpressing mice exhibited increased IgG deposition in the kidney and primarily localized along the glomerular basement membrane.

IgG Antibody Information：Leica DS9800

Fig.6 Renal PSA staining and pathological analysis. 

hPLA2R1-overexpressing mice exhibited evident kidney injury, including interstitial damage, tubular dilation, cast formation, and basement membrane thickening. (n=2/group/timepoint).