Apc-L850X

品系全名

C57BL/6JSmo-Apcem1(L850X)Smoc

目录号

NM-KI-200001

品系状态

活体

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基因信息

基因名
Apc

基因曾用名

CC1， Min， mAPC， AI047805， AU020952， AW124434

NCBI ID

11789

MGI ID

88039

Ensembl ID

ENSMUSG00000005871

Pubmed

Apc

人类同源基因

APC

品系描述

家族性腺瘤性息肉病（familial adenomatous polyposis, FAP）是结直肠癌发生的癌前病变，患者直肠部位多发成百上千个腺瘤性息肉，抑癌基因APC的突变是结直肠肿瘤的起始因素。1990年建立的C57BL/6-APCmin/+小鼠品系是在小鼠同源基因Apc基因第850位点氨基酸发生无义突变，造成其肠道多发腺瘤，被认为是一种良好的FAP小鼠模型。 将小鼠Apc基因850位氨基酸L替换为X（终止突变），建立该Apc基因点突变小鼠模型。该模型纯合子不能存活，验证结果显示，该模型是理想的肠道肿瘤模型。
应用领域：肠道肿瘤

疾病预测

Lynch综合征
Lynch Syndrome

近似模型的表型

MGI:4412021
注：该品系需与Mlh1-KO(NM-KO-190626)交配才可能获得预期表型

参考文献

Edelmann W, Yang K, Kuraguchi M, Heyer J, Lia M, Kneitz B, Fan K, Brown AM, Lipkin M, Kucherlapati R, Tumorigenesis in Mlh1 and Mlh1/Apc1638N mutant mice. Cancer Res. 1999 Mar 15;59(6):1301-7

大肠癌
Colorectal Cancer

近似模型的表型

MGI:5636659
注：该品系需与Msh2-KO(NM-KO-2113943)交配才可能获得预期表型

参考文献

Reitmair AH, Cai JC, Bjerknes M, Redston M, Cheng H, Pind MT, Hay K, Mitri A, Bapat BV, Mak TW, Gallinger S, MSH2 deficiency contributes to accelerated APC-mediated intestinal tumorigenesis. Cancer Res. 1996 Jul 1;56(13):2922-6

大肠癌
Colorectal Cancer

近似模型的表型

MGI:5693797
注：该品系需与Ccdc80-KO(NM-KO-200795)交配才可能获得预期表型

参考文献

Grill JI, Neumann J, Herbst A, Hiltwein F, Ofner A, Marschall MK, Wolf E, Kirchner T, Goke B, Schneider MR, Kolligs FT, DRO1 inactivation drives colorectal carcinogenesis in ApcMin/+ mice. Mol Cancer Res. 2014 Nov;12(11):1655-62

家族性腺瘤性息肉病
Familial Adenomatous Polyposis

近似模型的表型

MGI:4440863
注：该品系需与Rab25-KO(NM-KO-191148)交配才可能获得预期表型

参考文献

Nam KT, Lee HJ, Smith JJ, Lapierre LA, Kamath VP, Chen X, Aronow BJ, Yeatman TJ, Bhartur SG, Calhoun BC, Condie B, Manley NR, Beauchamp RD, Coffey RJ, Goldenring JR, Loss of Rab25 promotes the development of intestinal neoplasia in mice and is associated with human colorectal adenocarcinomas. J Clin Invest. 2010 Mar;120(3):840-9

验证数据

小鼠生存率分析结果显示， Apc-(L850X)突变小鼠通常会在160天前死亡（Figure 1）。

图片1.png

Figure 1. Survival Percent Of MT and WT Mouse(n=24).


小鼠摄食量和体重分析结果显示， Apc-(L850X)突变小鼠随着周龄增加摄食量减少，体重也偏轻（Figure 2-3）。

apc-2-3.png

在Apc-(L850X)突变小鼠的肠道中，随着周龄增加肿瘤数量也逐渐增加（ Figure 4-5 ）。

apc-4.png

Figure 4. Intestinal Tract Tumour Numbers of MT and WT Mouse.

image.png

Figure 5. Mouse Intestinal Tract on day 109


病理检测结果显示，Apc-(L850X)突变小鼠的肠道肠上皮可见腺癌组织增生（Figure 6-8）。蓝色箭头指示细胞核深染，腺体增生，为肠腺瘤；黄色箭头为炎症细胞浸润粘膜层；灰色箭头为肠系膜淋巴结。

图片1.png

Figure 6: Histological Result of WT and MT Mouse jejunum.

图片2.png

Figure 7: Histological Result of WT and MT Mouse ileum.

图片3.png

Figure 8: Histological Result of WT and MT Mouse colon.


发表文献 1篇

1. Ribonuclease 5/Angiogenin Suppresses Intestinal Tumor Initiation by Maintaining Crypt Homeostasis
发表年份：2026 来源杂志：CANCER RESEARCH

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