hHER2
品系全名
C57BL/6Smoc-Erbb2em1(hERBB2)Smoc
目录号
NM-HU-200115
品系状态
活体
基因信息
基因名
Erbb2
品系描述
通过同源重组，将小鼠Erbb2基因进行人源化修饰。
应用领域：免疫相关；肿瘤研究；药物筛选
*使用本品系发表的文献需注明: hHER2 mice (Cat. NO. NM-HU-200115) were purchased from Shanghai Model Organisms Center, Inc..
验证数据
Fig1. Detection of HER2 expression by RT-PCR.
Wild type: only one band at 375 bp with primers F1/R1 (mHer2);
Homozygous: one band at 223 bp with primers F2/R2 (hHER2).
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig2. Detection of HER2 expression in homozygous hHER2 mice by Western Blot.
(Note: The HER2 antibody cross-reacted with mHER2 and humanized HER2)
Fig.3 In vivo efficacy assessment of Herceptin in hHER2 mice bearing B16F10-HER2 syngeneic tumor.
Body weight (A) and Tumor volume (B) were measured of hHER2 mice.
你也可能感兴趣
Tamoxifen诱导Cre-ERT2小鼠 使用指南
Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。
查看
