每年的5月10日是“世界狼疮日”，主题是——Make Lupus Visible · 让狼疮显现。这一天，是为了让全球更多人关注这种被称为“千面疾病”的复杂病症——系统性红斑狼疮。

系统性红斑狼疮是一种复杂且异质性很高的自身免疫病，多发于育龄期女性，其根本原因是免疫系统的紊乱。在遗传易感性与环境因素（如紫外线、病毒感染）的交互作用下，机体免疫耐受崩溃，导致自身反应性T细胞和B细胞异常活化、致病性自身抗体大量产生及免疫复合物在全身多脏器沉积。系统性红斑狼疮被称为“伟大的模仿者”，该病可模拟多种疾病，临床表现多样且多变，可引起狼疮性肾炎、关节炎、皮肤蝶形红斑、中枢神经系统损害乃至溶血性贫血。

图. 免疫激活物质的过度生产导致SLE发病的作用机制^[1]

诱导模型

南模生物可提供Pristane诱导的BALB/c、C57BL/6系统性红斑狼疮模型，部分数据如下：

基于上述原理，南模生物通过肾切+Pristane+IMQ诱导构建了SLE模型，并且在不同品系（hCD3EDG/hCD19，BALB/c等）中进行了表型验证，该模型发病快，大大缩短了造模时间，表型明显，可用于SLE抗体类药物的药效评价。部分数据如下：

Fig.1 IMQ and Pristane induce nephrectomy Balb/c mice SLE model. (A) Body weight. (B) Body weight change.

Fig.2 Serum anti-dsDNA IgG Ab of IMQ and Pristane induce nephrectomy Balb/c mice SLE model.

Fig.3 IMQ and Pristane induce nephrectomy Balb/c mice SLE model. (A) mCD45⁺ cells in live cells proportion. (B) mCD20⁺ cells in live cells proportion. (C) mCD20⁺ cells in mCD45⁺ cells proportion.

Fig.4 IMQ and Pristane induce nephrectomy Balb/c mice SLE model. (A) Urine Albumin. (B) Urine Creatinine. (C) UACR (Albumin to creatinine ratio).

Fig.5 IMQ and Pristane induce nephrectomy Balb/c mice SLE model. (A) Spleen index. (B) Kidney index.

Fig.6 IMQ and Pristane induce nephrectomy Balb/c mice SLE model. (A) Representative H&E staining. (B) Histopathology score.

Fig.7 IMQ and Pristane induce nephrectomy Balb/c mice SLE model. (A) mIgG IHC staining. (B) % mIgG positive cells.

Fig.1 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Body weight. (B) Body weight change.

Fig.2 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Serum BUN. (B) Serum CRE. (*P<0.05, **P<0.01 Vs Group2)

Fig.3 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Serum anti-dsDNA IgG Ab on Day 28. (B) Serum anti-dsDNA IgG Ab on Day 68.

Fig.4 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) mCD45⁺ cells in live cells proportion. (B) mCD20⁺ cells in live cells proportion. (C) mCD20⁺ cells in mCD45⁺ cells proportion.

Fig.5 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Urine Albumin. (B) Urine Creatinine. (C) UACR (Albumin to creatinine ratio). (*P<0.05,**P<0.01 Vs Group2)

Fig.6 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Spleen photo and Kidney photo. (B) Spleen index and Kidney index.  (**P<0.01 Vs Group2)

Fig.7 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Representative H&E images in SLE model. (B)Histopathology score. (*P<0.05 Vs Group2)

Fig.8 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Representative IHC images in SLE model. (B) % mIgG positive cells.

Fig.1 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Serum anti-dsDNA IgG Ab. (B) Urine Albumin. (C) Urine Creatinine. (D) Albumin to creatinine ratio.

Fig.2 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. (A) Representative H&E images in SLE model. (A) Representative IHC images in SLE model. (C)Histopathology score. (D) % mIgG positive cells.

Fig.3 IMQ and Pristane induce nephrectomy hCD3EDG/hCD19 mice SLE model. Flow cytometric quantification of lymph (A) mCD45⁺, (B) hCD3⁺, (C) mCD4⁺, (D) mCD8⁺, (E) Treg cells, (F) hCD19⁺ B cells, (G) Dendritic cells (DC), and (H) mCD11b⁺ myeloid cells.

人源化SLE模型