Cdh5-2A-CreERT2
品系全名
C57BL/6Smoc-Cdh5em1(2A-CreERT2-WPRE-polyA)Smoc
目录号
NM-KI-200173
品系状态
活体
基因信息
基因名
Cdh5
品系描述
验证数据

Fig. 1 CreERT2-mediated recombination in the aorta of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the arterial endothelial cells of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

Fig. 2 CreERT2-mediated recombination in the spinal cord of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the endothelial cells derived from spinal cord of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

Fig. 3 CreERT2-mediated recombination in the cerebellum of Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in some cells of cerebellum derived from Cdh5CreERT2 /+; Rosa26tdTomato/+ mouse after tamoxifen treatment.

Fig. 4 Detection of tdTomato(red) in various tissues of Cdh5CreERT2/+; Rosa26tdTomato/+ mice. CreERT2 mediated recombination can be detected in the brain, pituitary gland, eyes, heart, lung, liver, submandibular gland, kidney, pancreas, testis, muscle, intestine, colon, spleen, thymus and brown fat. TdTomato expression can not be observed in the epidermis. (For more detailed information please contact our technical advisor.)
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Cre-ERT2在无Tamoxifen诱导的情况下,在细胞质内处于无活性状态;当Tamoxifen诱导后,Tamoxifen的代谢产物4-OHT(雌激素类似物)与ERT结合,可使Cre-ERT2进核发挥Cre重组酶活性。
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