Axin2-CreERT2

A CreERT2-pA expression cassette was knocked into the Axin2 gene start codon site. As Axin2 is an intracellular protein, it is essential for identifying a surface marker for stem cell purification. When these Axin2-CreERT2 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in Axin2 expressing cells.

Fig. 1 CreERT2-mediated recombination in the villi and crypts of small Intestine of Prom1-CreERT2; Rosa26-tdTomato mice after tamoxifen treatment.

Fig. 2 CreERT2-mediated recombination in the alveoli of Axin2-CreERT2; Rosa26-tdTomato mice after tamoxifen treatment.

Fig.3 Tdtomato was also abundantly expressed in brain, large intestine, small intestine, kidney, liver, lung, ovary (leaky  expression), and uterus (leaky expression);  expressed in brown fat, eye, heart valve, muscle, pancreas, stomach and thymus (leaky expression). There was a small amount of expression in salivary glands and spleen.（For more information please contact: 400-728-0660.）