Apc-L850X

Familial adenomatous polyposis (FAP) is a heritable condition in which affected individuals develop benign intestinal polyps. Often, polyps progress to colorectal carcinomas later in life. The mutation of C57BL/6-Apcem1(L850X)Smoc(NM-KI-200001) strain of SMOC refers to the ApcMin model (Jax: 002020 ), a useful model to study intestinal adenomas. The C57BL/6-Apcem1(L850X)Smoc strain is highly susceptible to spontaneous intestinal adenoma formation and can be used as a research model for human FAP. These mice carry a p.L850X mutation of Apc gene.

Along with the development of intestinal adenoma, we found that most  of Apc-(L850X) mice heterozygous mice raised on a normal diet  died  by 160 days of age (Figure 1).

Figure 1. Survival Percent Of MT and WT Mouse(n=24).

The data of food intake and body weight analysis showed that Apc-(L850X) mice had reduced food intake and lighter body weights with the age(Figure 2-3).

Most of the C57BL/6-Apcem1(L850X)Smoc heterozygous mice raised adenomas throughout the intestinal tract with the age (Figure 4-5 ).

Figure 4. Intestinal Tract Tumour Numbers of MT and WT Mouse.

Figure 5. Mouse Intestinal Tract on day 109.

Pathologic results showed that adenomatous hyperplasia appeared in epithelium of intestinal type (Figure 6-8). Blue arrows indicate hyperplasia of gland, which is intestinal adenoma. Yellow arrows show inflammatory cells infiltrating the mucosal layer. Gray arrows show mesenteric lymph nodes.

Figure 6: Histological Result of WT and MT Mouse jejunum.

 

Figure 7: Histological Result of WT and MT Mouse ileum.

Figure 8: Histological Result of WT and MT Mouse colon.