hTGFBR2
品系全名
C57BL/6JSmo-Tgfbr2tm1(hTGFBR2)Smoc
目录号
NM-HU-252006
品系状态
活体
基因信息
基因名
Tgfbr2
品系描述
验证数据
Fig.1 Detection of TGFBR2 expression in subcutaneous fat pad and lung by RT-PCR. Mouse Tgfbr2 mRNA (145 bp) was detectable only in wild-type C57BL/6 mice. Human TGFBR2 mRNA (319 bp/429 bp) was detectable only in homozygous hTGFBR2 knockin mice but not in wild-type mice. Subcutaneous fat pad and lung RNA was extracted from 7-week-old male wild-type C57BL/6 (WT)(n=1) and homozygous hTGFBR2 knockin mice (HO) (n=2), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mRNA primers.
Abbr. M, marker; HO, homozygous; WT, wild type.
Fig.2 Detection of human TGFBR2 expression on CD3+ T cells in blood and spleen of WT C57BL/6 mice and HO hTGFBR2 mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (female, 6.4 weeks old) and homozygous hTGFBR2 humanized mice (female, 6.4 weeks old), and analyzed by flow cytometry with anti-hTGFBR2.
Abbr. HO, homozygous; WT, wild type.
Fig.3 Detection of mouse TGFBR2 expression on CD3+ T cells in blood and spleen of WT C57BL/6 mice and HO hTGFBR2 mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (female, 6.4 weeks old) and homozygous hTGFBR2 humanized mice (female, 6.4 weeks old), and analyzed by flow cytometry with anti-hTGFBR2.
Abbr. HO, homozygous; WT, wild type.
Fig.4 Detection of human TGFBR2 expression on CD4+ T cells in blood and spleen of WT C57BL/6 mice and HO hTGFBR2 mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (female, 6.4 weeks old) and homozygous hTGFBR2 humanized mice (female, 6.4 weeks old), and analyzed by flow cytometry with anti-hTGFBR2.
Abbr. HO, homozygous; WT, wild type.
Fig.5 Detection of mouse TGFBR2 expression on CD4+ T cells in blood and spleen of WT C57BL/6 mice and HO hTGFBR2 mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (female, 6.4 weeks old) and homozygous hTGFBR2 humanized mice (female, 6.4 weeks old), and analyzed by flow cytometry with anti-hTGFBR2.
Abbr. HO, homozygous; WT, wild type.
Fig.6 Detection of human TGFBR2 expression on CD19+ B cells in blood and spleen of WT C57BL/6 mice and HO hTGFBR2 mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (female, 6.4 weeks old) and homozygous hTGFBR2 humanized mice (female, 6.4 weeks old), and analyzed by flow cytometry with anti-hTGFBR2.
Abbr. HO, homozygous; WT, wild type.
Fig.7 Detection of mouse TGFBR2 expression on CD19+ B cells in blood and spleen of WT C57BL/6 mice and HO hTGFBR2 mice by FACS.
Leukocytes were collected from wild-type C57BL/6 mice (female, 6.4 weeks old) and homozygous hTGFBR2 humanized mice (female, 6.4 weeks old), and analyzed by flow cytometry with anti-hTGFBR2.
Abbr. HO, homozygous; WT, wild type.
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