Ace2-2A-CreERT2

A 2A-CreERT2-WPRE-polyA expression cassette was knocked into the Ace2 gene stop codon site. Ace2 encodes angiotensin I converting enzyme 2. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing Cre. This strain is useful for studying lung injury and kidney disease caused by SARS coronavirus infection.

Fig. 1 CreERT2-mediated recombination in the lung of Ace2^CreERT2/+; Rosa26^tdTomato/+ mouse. TdTomato(red) expression can be detected in the pulmonary bronchial epithelial cells of Ace2^CreERT2/+; Rosa26^tdTomato/+ mouse after tamoxifen treatment. 

Fig. 2 CreERT2-mediated recombination in the kidney of Ace2^CreERT2/+; Rosa26^tdTomato/+ mouse. TdTomato(red) expression can be detected in the proximal tubule of Ace2^CreERT2/+; Rosa26^tdTomato/+ mouse after tamoxifen treatment.