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Apoe-KO

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Strain Information

Strain Name

SD-Apoe^em1Smoc

Cat. NO.

NR-KO-190003

Gene Symbol

Apoe

Gene Synonyms

apo-E

Human Ortholog

APOE

Ensembl ID

ENSRNOG00000018454

MGI ID

NCBI ID

25728

Status

Repository Live

Research Application

动脉粥样硬化、高脂血症、血胆脂醇过多、心脏功能损伤、脑梗塞、老年痴呆症及慢性乙型肝炎等

Description

利用CRISPR基因编辑技术，敲除Apoe基因Exon 2-4，建立Apoe基因敲除大鼠模型。

Validation Data

图1. RT-PCR检测Apoe mRNA在WT和KO大鼠品系外周血中的表达。结果证明：纯合子KO大鼠在外周血中未检测到Apoe的表达，敲除效果良好。A图引物对针对大鼠Apoe cDNA序列（一条引物在敲除区域），B图引物对针对内参actin序列。HE(杂合子）和WT(野生型）大鼠用Apoe引物对可扩增出1044bp产物，HO（纯合子）无条带。M:100bp Plus DNA ladder from Transgen (BM311)

Publications

Related Strain

Cdh18-Flox Strain Name：C57BL/6-Cdh18^{em1(flox)Smoc} Status：Developing \| Cat. NO.：NM-CKO-2103934
Cdh18-KO Strain Name：C57BL/6-Cdh18^em1Smoc Status：Developing \| Cat. NO.：NM-KO-2101190
Lrp8-Flox Strain Name：C57BL/6-Lrp8^{em1(flox)Smoc} Status：Developing \| Cat. NO.：NM-CKO-2117157
Lrp8-KO Strain Name：C57BL/6-Lrp8^em1Smoc Status：Embryo cryopreservation \| Cat. NO.：NM-KO-190830

Cdh18-Flox Strain Name：C57BL/6-Cdh18^{em1(flox)Smoc} Status：Developing \| Cat. NO.：NM-CKO-2103934
Cdh18-KO Strain Name：C57BL/6-Cdh18^em1Smoc Status：Developing \| Cat. NO.：NM-KO-2101190
Lrp8-Flox Strain Name：C57BL/6-Lrp8^{em1(flox)Smoc} Status：Developing \| Cat. NO.：NM-CKO-2117157
Lrp8-KO Strain Name：C57BL/6-Lrp8^em1Smoc Status：Embryo cryopreservation \| Cat. NO.：NM-KO-190830

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