Humanized Mice

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• HSC Humanized Mice
• PBMC Humanized Mice

General information

PBMC humanized mice are effective model animals for infectious diseases, GvHD, and immuno-oncology. In case of stimulation by mouse xenoantigens, T cells in human-derived PBMCs can proliferate, while other types of cells maintain a low content or die prematurely. The human-derived cells in PBMC humanized mice are mainly T cells, which are suitable for various studies that require T cell immune responses. However, T cells also over-immunize the recipient mice and then trigger graft-versus-host disease (GvHD), which may cause the mice to die after a few weeks. Therefore, PBMC humanized mice have a short window period for tests and are only suitable for short-term studies. The PBMC humanized mice are constructed on the basis of NMG-B2m mice but experience the elimination of the B2M β-subunit gene of the MHC class I molecule, which will delay the attack of human immune cells against the host and extend the test window period.

Preparation process

Figure 1. Preparation process of NMG mice transplanted with human-derived PBMCs.

Phenotypic analysis

Figure 2. Human-derived CD45+ cell content in peripheral blood of NMG mice transplanted with human-derived PBMCs and body weight changes of mice.

Six groups of NMG mice were injected with PBMCs from six donors via tail vein, respectively. The overall trend of increased proportion of human-derived CD45+ cells could be detected, with differences in chimerism proportion changes but overall trend as expected. Relatively stable changes in body weight were observed in the mice.

Efficacy evaluation

Figure 3. Anti-tumor efficacy validation in Hu-PBMC H929 tumor-bearing mouse model

Figure 4. Anti-tumor efficacy validation in Hu-PBMC A431 tumor-bearing mouse model

Application field

• Immuno-oncology study

• GvHD study

• Hematological disease study

• Infectious disease study

• Gene therapy

• Study on drug targets without cross reactions

• Immunogenicity assay

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