hEPCAM

品系全名

C57BL/6Smoc-EpCAMtm1(hEpCAM)Smoc

目录号

NM-HU-210032

品系状态

精子冻存

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基因信息

基因名
Epcam

基因曾用名

EGP; Ly74; gp40; CD326; EGP-2; TROP1; Egp314; Ep-CAM; EpCAM1; Tacsd1; GA733-2; Tacstd1

NCBI ID

17075

MGI ID

106653

Ensembl ID

ENSMUSG00000045394

Pubmed

Epcam

人类同源基因

EPCAM

品系描述

利用ES细胞打靶方式，将小鼠Epcam基因全部或者部分替换为人源EPCAM。从而表达人或人鼠嵌合EPCAM蛋白，取代小鼠内源Epcam蛋白的表达。
应用领域：免疫治疗；药物筛选

验证数据

1.png

Fig1. Analysis of mouse EPCAM and human EPCAM protein expression in the large intestine by IHC. Both the EPCAM humanized heterozygous mouse and wild type express mouse EPCAM in the mucosal epithelial membranes, and the humanized heterozygous mouse  expresses human EPCAM in the crypts significantly.

2.pngFig2. Analysis of mouse EPCAM and human EPCAM protein expression in the small intestine by IHC. Both the EPCAM humanized heterozygous mouse and wild type express mouse EPCAM in the mucosal epithelial membranes, and the humanized heterozygous mouse  expresses human EPCAM in the crypts significantly.

image.png

Fig3. Detection of human EpCAM expression in WT C57 and homozygous hEpCAM mice by IHC. Small intestine were collected from wild-type mice (+/+) and homozygous hEpCAM mice (HO/HO), and analyzed by IHC with anti-human EpCAM antibody. 

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