Cd4-IRES-DTRGFP

Fig.1 Expression analysis of CD4 and GFP on CD3+T in blood of CD4-IRES-DTRGFP mice.

Leukocytes were collected from the blood of 9-week-old CD4-IRES-DTRGFP mice, and analyzed by flow cytometry. Most CD4+T cells (97.3%) were GFP-positive cells.

Fig.2 CD4+ T cell and various immune cell populations in peripheral blood of CD4-IRES-DTR-EGFP mice. (In collaboration with CrownBio)

C57BL/6 mice and CD4-IRES-DTR-EGFP mice were simultaneously injected with diphtheria toxin. Results showed that after injection, CD4+ T cells and Treg cells in peripheral blood of CD4-IRES-DTR-EGFP mice were gradually depleted to 0%, while the proportion of CD8+ T cells in peripheral blood relatively increased. In contrast, immune cells such as B cells, NK cells, NKT cells, and DCs exhibited similar trends in peripheral blood between CD4-IRES-DTR-EGFP mice and wild-type mice.

Fig.3 Tumor growth in CD4-IRES-DTR-EGFP heterozygous MC38 tumor-bearing mice at different time points. (In collaboration with CrownBio)

After CD4-IRES-DTR-EGFP heterozygous mice and wild-type mice were inoculated with the MC38 colon cancer cell line, diphtheria toxin was injected on day 8 and subsequently administered every two days for a total of 3 doses. Tumor growth was monitored before and after treatment.

Different syngeneic tumors show distinctive intrinsic tumor-immunity and mechanisms of actions (MOA) of anti-PD-1 treatment
来源杂志：Scientific reports