hIL2/hIL2RA(BALB/c)
品系全名
BALB/cAnSmoc-Il2tm3(hIL2)Il2ratm1(hIL2RA)/Smoc
目录号
NM-HU-220018
品系状态
胚胎冻存
品系描述
The endogenous mouse Il2 and Il2ra gene was replaced respectively by human IL2 and IL2RA gene.
*使用本品系发表的文献需注明: hIL2/hIL2RA(BALB/c) mice (Cat. NO. NM-HU-220018) were purchased from Shanghai Model Organisms Center, Inc..
验证数据
Fig1. Detection of IL2 expression in splenocyte by RT-PCR.
Wild type: only one band at 288 bp with primers F1/R1(mIl2);
Homozygous: only one band at 388 bp with primers F2/R2(hIL2);
Abbr. M, DNA marker; HO, homozygous; WT, wild type.
Fig2. Detection of IL2RA expression in splenocyte by RT-PCR.
Wild type: only one band at 350 bp with primers F1/R1(mIl2ra);
Homozygous: only one band at 318 bp with primers F2/R2(hIL2RA);
Abbr. M, DNA marker; HO, homozygous;WT, wild type.
Fig3. Detection of hIL2RA expression on splenocyte-derived T cells in hIL2/hIL2RA dKI mice.
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