Nkx2-5-IRES-Cre

品系全名

C57BL/6Smoc-Nkx2-5em1(IRES-Cre-WPRE-polyA)Smoc

目录号

NM-KI-200152

品系状态

活体

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基因信息

基因名
Nkx2-5

基因曾用名

Csx; Nkx2.5; tinman; Nkx-2.5

NCBI ID

18091

MGI ID

97350

Ensembl ID

ENSMUSG00000015579

Pubmed

Nkx2-5

人类同源基因

NKX2-5

品系描述

A IRES-Cre-WPRE-polyA expression cassette was knocked into the Nkx2-5 gene stop codon site.
应用领域：Cre recombinase tool; Nkx2.5 acts as a key transcription factor and plays essential roles for heart formation. By mating the reporter mice with Cre-expressing mice, Nkx2-5 positive cells derived from double-positive mice were permanently labeled by fluorescent protein. When crossed with mice carrying a targeted floxed alleles, this strain is useful in eliminating tissue-specific conditional expression of the gene.

验证数据

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Fig. 1 Cre-mediated recombination in the heart of Nkx2-5Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the myocardial cells and vascular cells of Nkx2-5Cre/+; Rosa26tdTomato/+ mouse.

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Fig. 2 Detection of tdTomato(red) in various tissues of Nkx2-5Cre/+; Rosa26tdTomato/+ mice. Cre mediated recombination can be detected in the heart, bronchus, pulmonary alveoli and submandibular gland. Tdtomato expression can be also detected in individual cells derived from the brain, retina, skin, kidney, pancreas, intestine, colon, spleen and thymus, except for pituitary and muscle. (For more detailed information please contact our technical advisor.)


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