Nkx2-1-CreERT2

A CreERT2-pA expression cassette was knocked into the Nkx2-1 gene start codon site. Nkx2-1, a key molecule in lung development, is expressed in adult bronchial and alveolar type Ⅱ epithelial cells. Besides, Nkx2-1 expression as a prognostic marker is also been applied to lung cancer research including lung adenocarcinoma.

Fig. 1 Detection of tdTomato (red) in the pulmonary epithelial cells of Nkx2-1CreERT2/+; Rosa26tdTomato/+  mouse after tamoxifen treatment. CreERT2-mediated recombination in some of the bronchial and alveolar epithelial cells of Nkx2-1CreERT2/+; Rosa26tdTomato/+ mouse can be induced by tamoxifen. 

Fig. 2 Detection of tdTomato (red) in various tissues of Nkx2-1CreERT2/+; Rosa26tdTomato/+ mice after tamoxifen treatment. CreERT2-mediated recombination in the lung can be induced by tamoxifen. And tdTomato expression can not be observed in the pituitary gland, retina, heart, liver, stomach, colon,  intestine, kidney, pancreas, etc.