Nos1-IRES-Cre

品系全名

C57BL/6Smoc-Nos1em1(IRES-iCre)Smoc

目录号

NM-KI-200113

品系状态

精子冻存

导出PDF

基因信息

基因名
Nos1

基因曾用名

NO; NOS; bNOS; nNOS; N-NOS; NOS-I; Nos-1; NC-NOS; 2310005C01Rik

NCBI ID

18125

MGI ID

97360

Ensembl ID

ENSMUSG00000029361

Pubmed

Nos1

人类同源基因

NOS1

品系描述

A IRES-iCre expression cassette was knocked into the Nos1 gene stop codon site.
应用领域：Cre recombinase tool; NOS1, the neurogenic nitric oxide synthase, is a key enzyme responsible for the production of the signaling molecule nitric oxide (NO) by neurons. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre. This strain may be useful for studying diseases with impaired or reduced nitric oxide release, such as achalasia of cardia, hirschsprung's disease, hypertrophic pyloric stenosis, etc.

验证数据

image.png

Fig. 1 Cre-mediated recombination in the brain of Nos1Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the olfactory bulb, cortex and hippocampus derived from Nos1Cre/+; Rosa26tdTomato/+ mouse.

image.png

Fig. 2 Cre-mediated recombination in the large intestine of Nos1Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the muscular layer and mucous layer of the wall of the large intestine derived from Nos1Cre/+; Rosa26tdTomato/+ mouse.

image.png

Fig. 3 Detection of tdTomato(red) in various tissues of Nos1Cre/+; Rosa26tdTomato/+ mice. Cre mediated recombination can be detected in  the brain, eyes, pituitary gland, heart, liver, stomach, brown fat, kidney, pancreas, thymus, colon, spleen, skin, muscle, uterus, ovary and submandibular gland. (For more detailed information please contact our technical advisor.)


你也可能感兴趣

Tamoxifen诱导Cre-ERT2小鼠 使用指南

Cre-ERT2在无Tamoxifen诱导的情况下，在细胞质内处于无活性状态；当Tamoxifen诱导后，Tamoxifen的代谢产物4-OHT（雌激素类似物）与ERT结合，可使Cre-ERT2进核发挥Cre重组酶活性。

查看
【小鼠大学问】基因工程小鼠的命名规则

常见的基因工程小鼠可以分为两种命名方式，包括基因定点修饰的小鼠命名，比如：敲除、敲入、点突变等等，和随机转基因的小鼠命名。

查看