Flt3-KO(M-NSG)

品系全名

NOD.Cg-PrkdcscidIl2rgem1Flt3em1Smoc

目录号

NM-KO-234079

品系状态

活体

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基因信息

基因名
Flt3

基因曾用名

Flk2; Ly72; wmfl; CD135; Flk-2; Flt-3; B230315G04

NCBI ID

14255

MGI ID

95559

Ensembl ID

ENSMUSG00000042817

Pubmed

Flt3

人类同源基因

FLT3

品系描述

通过敲除Flt3基因exon3，建立Flt3基因敲除小鼠模型。

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验证数据

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Fig.1 Expression analysis of Flt3 on CD117+ cells or CD3-CD19-NK1.1-CD11b-Gr1-F4/80-CD11c- (lineage-negative) cells in bone marrow of M-NSG mice and Flt3-KO(M-NSG) mice.

Leukocytes were collected from bone marrow of 8-week-old M-NSG mice and Flt3-KO(M-NSG) mice. Flt3 was detectable on both CD117+ cells and lineage-negative cells of M-NSG mice, whereas its expression was  lost in the corresponding subsets of Flt3-KO(M-NSG) mice.

image.png

Fig.2 Expression analysis of Flt3 on cDCs and pDCs of M-NSG mice and Flt3-KO(M-NSG) mice.

Leukocytes were collected from spleen of 8-week-old M-NSG mice and Flt3-KO(M-NSG) mice. Flt3 was readily detected on both cDCs and pDCs of M-NSG mice, whereas its expression was lost in the corresponding subsets of Flt3-KO(M-NSG) mice.

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Fig.3 Analysis of cDCs (CD11c+PDCA1-) and pDCs (CD11c+PDCA1+) in CD45+ cells in bone marrow of M-NSG mice and Flt3-KO (M-NSG) mice.

Leukocytes were collected from bone marrrow of 8-week-old M-NSG mice and Flt3-KO (M-NSG) mice. Both cDCs and pDCs were decreased in bone marrow of Flt3-KO(M-NSG) mice.

image.png

Fig.4 Analysis of cDCs (CD11c+PDCA1-) and pDCs (CD11c+PDCA1+) in CD45+ cells in spleen of M-NSG mice and Flt3-KO(M-NSG) mice.

Leukocytes were collected from spleen of 8-week-old M-NSG mice and Flt3-KO(M-NSG) mice. Both cDCs and pDCs were decreased in spleen of Flt3-KO(M-NSG) mice.

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