hACE2

品系全名

C57BL/6JSmo-Ace2tm3(hACE2-flag-Wpre-pA)Smoc

目录号

NM-HU-200218

品系状态

胚胎冻存

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基因信息

基因名
ACE2

基因曾用名

ACEH

NCBI ID

59272

MGI ID

Null

Ensembl ID

ENSG00000130234

Pubmed

ACE2

人类同源基因

ACE2

品系描述

利用CRISPR基因编辑技术，将人源ACE2-flag-Wpre-pA共表达结构替换小鼠Ace2基因起始密码子处，从而表达人ACE2蛋白，取代小鼠内源Ace2蛋白的表达。
应用领域：新型冠状病毒疾病（COVID-19）等研究

验证数据

图1. qPCR检测ACE2人源化小鼠各组织中hACE2的表达情况。 结果显示ACE2人源化杂合子和纯合子小鼠中肺、肾、小肠中可以检测到人源ACE2的显著表达；野生型小鼠和杂合子小鼠心、肺、肾、小肠中可以检测到鼠源Ace2的显著表达，纯合子小鼠各组织中均检测不到鼠源Ace2的明显表达。

hACE2-3.png

图2. RT-PCR检测ACE2人源化小鼠肺中ACE2的表达情况。引物对P7/8特异针对446bp的鼠源Ace2序列;引物对P5/6特异针对283bp的人源ACE2序列；M:100bp Plus DNA ladder from Transgen (BM311)。结果表明，纯合子小鼠（HO）肺部只表达人源ACE2，杂合子小鼠（HE）肺部同时表达人源ACE2和鼠源Ace2，野生型小鼠（WT）肺部只表达鼠源Ace2。

发表文献 4篇

1. The Abundant Distribution and Duplication of SARS-CoV-2 in the Cerebrum and Lungs Promote a High Mortality Rate in Transgenic hACE2-C57 Mice
发表年份：2024-01-13 来源杂志：International Journal of Molecular Sciences

2. TRIM28-mediated nucleocapsid protein SUMOylation enhances SARS-CoV-2 virulence
发表年份：2024 来源杂志：Nature Communications

3. A two-adjuvant multiantigen candidate vaccine induces superior protective immune responses against SARS-CoV-2 challenge
发表年份：2021 来源杂志：Cell Reports

4. Specific inhibition of the NLRP3 inflammasome suppresses immune overactivation and alleviates COVID-19 like pathology in mice
发表年份：2021 来源杂志：EBioMedicine

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