Ace2-2A-CreERT2

品系全名

C57BL/6JSmo-Ace2em2(2A-CreERT2-WPRE-polyA)Smoc

目录号

NM-KI-200187

品系状态

精子冻存

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基因信息

基因名
ACE2

品系描述

将2A-CreERT2-WPRE-polyA插入到小鼠Ace2基因终止密码子处。 Ace2(angiotensin I converting enzyme (peptidyl-dipeptidase A) 2)血管紧张素转化酶2,当与含有 loxP 位点侧翼序列的小鼠品系杂交时,Cre 介导的重组导致后代在他莫昔芬诱导后的侧翼序列的缺失。可用于SARS冠状病毒感染的肺损伤和肾脏疾病的研究。
应用领域:Cre工具鼠

验证数据

image.png

图 1 Ace2CreERT2/+; Rosa26tdTomato/+小鼠肺部tdTomato的表达情况。

经他莫昔芬诱导后,Ace2CreERT2/+; Rosa26tdTomato/+双转基因鼠,可在小鼠肺支气管上皮细胞中检测到tdTomato的表达。

image.png

图 2 Ace2CreERT2/+; Rosa26tdTomato/+小鼠肾脏中tdTomato的表达情况。

经他莫昔芬诱导后,Ace2CreERT2/+; Rosa26tdTomato/+双转基因鼠,可在小鼠肾脏近端小管中检测到tdTomato的表达。

image.png

图 3 Ace2CreERT2/+; Rosa26tdTomato/+小鼠各组织中tdTomato的表达情况。他莫昔芬诱导后,TdTomato的表达可在小肠、大肠、肾脏、大脑、肺支气管上皮、胰腺、真皮和唾液腺中检测到。不表达的组织有:胸腺、眼球、心脏、肝、胃、棕色脂肪、脾脏、肌肉。(更多详细信息请联系我司技术顾问。)



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