Cdkn2a-2A-DreERT2(p16-2A-DreERT2)

Fig.1 Detection of Cdkn2a expression in kidney, lung, liver and skin by qPCR. Cdkn2a mRNA was detected at comparable levels and exhibited similar expression profiles in both wild-type and heterozygous Cdkn2a-2A-DreERT2 knockin mice, indicating that the knockin allele did not disrupt the endogenous expression pattern of the gene. Kidney, lung, liver and skin RNA was extracted from 9-week-old male wild-type C57BL/6 (WT)(n=3) and heterozygous Cdkn2a-2A-DreERT2 knockin mice (HO)(n=3), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with mouse Cdkn2a(F3/R3) and mouse Gapdh primers. Relative expression represents the mouse Cdkn2a(F3/R3) mRNA level relative to heterozygous Cdkn2a-2A-DreERT2 knockin mice expression in skin . 

Abbr. M, marker; HE, heterozygous; WT, wild type.

Fig.2 Detection of DreERT2 expression in kidney, lung, liver and skin by qPCR. DreERT2 mRNA was detectable in heterozygous Cdkn2a-2A-DreERT2 knockin mice but not in wild-type mice. Kidney, lung, liver and skin RNA was extracted from 9-week-old male wild-type C57BL/6 (WT)(n=3) and heterozygous Cdkn2a-2A-DreERT2 knockin mice (HO) (n=3), then cDNA libraries were synthesized by reverse transcription, followed by qPCR with DreERT2 and mouse Gapdh primers. Relative expression represents the DreERT2 mRNA level relative to heterozygous Cdkn2a-2A-DreERT2 knockin mice expression in skin.

Abbr. M, marker; HE, heterozygous; WT, wild type.