huHSC-(Kit-V831M M-NSG)

品系全名

huHSC-NOD.Cg-PrkdcscidIl2rgem1Kitem1(V831M)Smoc

目录号

NM-HU-263011

品系状态

活体

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品系描述

南模生物将小鼠Kit基因831位氨基酸由V替换为M构建了Kit-V831M(M-NSG)小鼠，并在此基础上将人CD34+ HSC移植入该小鼠体内。

验证数据

2f12f91d-ca9d-46ef-9f23-5a7f42a39843.png

Fig 1. Body weight growth curves of M-NSG and Kit-V831M(M-NSG) mice engrafted with human HSC. Human CD34+ HSCs  (1E5) were intravenously injected into homozygous Kit-V831M(M-NSG) and M-NSG mice (n=12-18, female, 4 weeks). Prior to inoculation, M-NSG mice were irradiated with 1.0 Gy, whereas Kit-V831M (M-NSG) mice received no irradiation.

c80386ef-2458-4d74-a45d-4c083edce1cd.png

Fig 2. Survival rates of M-NSG and Kit-V831M(M-NSG) mice engrafted with human HSC. Human CD34+ HSCs  (1E5) were intravenously injected into homozygous Kit-V831M(M-NSG) and M-NSG mice (n=6-7, female, 4 weeks). Prior to inoculation, M-NSG mice were irradiated with 1.0 Gy, whereas Kit-V831M (M-NSG) mice received no irradiation.

ea8b4739-127f-40a5-b8b4-5152c1282d53.png

Fig 3. Humanized immune reconstitution levels in M-NSG and Kit-V831M(M-NSG) mice engrafted with human HSC. Human CD34+ HSCs  (1E5) were intravenously injected into homozygous Kit-V831M(M-NSG) and M-NSG mice (n=12-18, female, 4 weeks). Prior to inoculation, M-NSG mice were irradiated with 1.0 Gy, whereas Kit-V831M (M-NSG) mice received no irradiation.

d3c12f60-591a-43a8-b2f0-8d3431daab06.png

Fig 4. Humanized immune reconstitution levels in M-NSG and Kit-V831M(M-NSG) mice engrafted with human HSC. Human CD34+ HSCs  (1E5) were intravenously injected into homozygous Kit-V831M(M-NSG) and M-NSG mice (n=12-18, female, 4 weeks). Prior to inoculation, M-NSG mice were irradiated with 1.0 Gy, whereas Kit-V831M (M-NSG) mice received no irradiation.

936c3f68-4f47-4f6f-8cc0-169bab58533c.png

Fig 5. Humanized immune reconstitution levels in M-NSG and Kit-V831M(M-NSG) mice engrafted with human HSC. Human CD34+ HSCs  (1E5) were intravenously injected into homozygous Kit-V831M(M-NSG) and M-NSG mice (n=12-18, female, 4 weeks). Prior to inoculation, M-NSG mice were irradiated with 1.0 Gy, whereas Kit-V831M (M-NSG) mice received no irradiation. At 26 weeks post immune reconstitution, bone marrow was collected from mice and human immune cell reconstitution levels were assessed by flow cytometry.

b34a5982-4381-4f93-b764-e57243285bb8.png

Fig 6. Humanized immune reconstitution levels in M-NSG and Kit-V831M(M-NSG) mice engrafted with human HSC. Human CD34+ HSCs  (1E5) were intravenously injected into homozygous Kit-V831M(M-NSG) and M-NSG mice (n=12-18, female, 4 weeks). Prior to inoculation, M-NSG mice were irradiated with 1.0 Gy, whereas Kit-V831M (M-NSG) mice received no irradiation. At 26 weeks post immune reconstitution, lymph node tissue was collected from mice and human immune cell reconstitution levels were assessed by flow cytometry.

e83ff29b-d673-49ed-8a54-e6183f320213.png

Fig 7. Humanized immune reconstitution levels in M-NSG and Kit-V831M(M-NSG) mice engrafted with human HSC. Human CD34+ HSCs  (1E5) were intravenously injected into homozygous Kit-V831M(M-NSG) and M-NSG mice (n=12-18, female, 4 weeks). Prior to inoculation, M-NSG mice were irradiated with 1.0 Gy, whereas Kit-V831M (M-NSG) mice received no irradiation. At 26 weeks post immune reconstitution, spleen tissue was collected from mice and human immune cell reconstitution levels were assessed by flow cytometry.


品系背景验证数据见 Kit-V831M(M-NSG) NM-KI-242813，点击下方链接了解详情：

Kit-V831M(M-NSG)-南模生物

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