CAG-hACE2-IRES-Luc-Tg

品系全名

C57BL/6JSmo-Tgtn(CAG-human ACE2-IRES-Luciferase-WPRE-polyA)Smoc

目录号

NM-TG-200002

品系状态

活体

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品系描述

该小鼠带有CAG启动子驱动的人源ACE2和Luciferase。
应用领域：新型冠状病毒疾病（COVID-19）等研究

验证数据


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图1 利用活体成像检测Tgtn(CAG-human ACE2-IRES-Luciferase)小鼠的Luciferase表达情况。

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图2 利用Western blot检测Tgtn(CAG-human ACE2-IRES-Luciferase)小鼠的人源 ACE2蛋白表达情况。

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图3. SARS-CoV-2感染后hACE2 Tg小鼠肺中的病毒滴度。 用相同剂量的SARS-CoV-2接种了hACE2 Tg小鼠和野生型小鼠，结果表明hACE2 Tg小鼠对SARS-CoV-2感染高度敏感。

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图4 给药后hACE Tg小鼠体重变化率

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图5 给药后hACE2 Tg小鼠肺部病毒载量变化

发表文献 13篇

1. STING agonist-based ER-targeting molecules boost antigen cross-presentation
发表年份：2025 来源杂志：NATURE

2. SARS-CoV-2 spike protein receptor-binding domain perturbates intracellular calcium homeostasis and impairs pulmonary vascular endothelial cells
发表年份：2023 来源杂志：Signal Transduction and Targeted Therapy

3. SARS-CoV-2 might transmit through the skin while the skin barrier function could be the mediator
发表年份：2022-01-03 来源杂志：MED HYPOTHESES

4. Broad neutralization of SARS-CoV-2 variants by an inhalable bispecific single-domain antibody
发表年份：2022 来源杂志：CELL

5. A novel STING agonist-adjuvanted pan-sarbecovirus vaccine elicits potent and durable neutralizing antibody and T cell responses in mice, rabbits and NHPs
发表年份：2022 来源杂志：CELL RESEARCH

6. Spike-mediated ACE2 down-regulation was involved in the pathogenesis of SARS-CoV-2 infection
发表年份：2022 来源杂志：JOURNAL OF INFECTION

7. Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2
发表年份：2022 来源杂志：Nature Microbiology

8. Comparison of model-specific histopathology in mouse models of COVID-19
发表年份：2022 来源杂志：JOURNAL OF MEDICAL VIROLOGY

9. Molecular imaging on ACE2-dependent transocular infection of coronavirus
发表年份：2022 来源杂志：JOURNAL OF MEDICAL VIROLOGY

10. hACE2 Fc-neutralization antibody cocktail provides synergistic protection against SARS-CoV-2 and its spike RBD variants
发表年份：2021-07-20 来源杂志：cell discovery

11. SARS-CoV-2 promotes RIPK1 activation to facilitate viral propagation
发表年份：2021 来源杂志：CELL RESEARCH

12. Structural and functional basis for pan-CoV fusion inhibitors against SARS-CoV-2 and its variants with preclinical evaluation
发表年份：2021 来源杂志：Signal Transduction and Targeted Therapy

13. Effects of simeprevir on the replication of SARS-CoV-2 in vitro and in transgenic hACE2 mice
发表年份：2021 来源杂志：INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS

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