pCD2-hTL1A-Tg

Fig.1 Detection of human TL1A expression in serum by ELISA (n=3, 6 weeks old). 

Abbr. WT, wild type; N.D. not detected.

Fig.2 Detection of Treg expression on CD3+T cells in MLN and spleen of WT (C57BL/6) mice and pCD2-hTL1A-Tg mice by FACS.

 In CD3+ T cells, compared with wild-type mice, the number of Treg cells in the spleen and MLN of pCD2-hTL1A-Tg mice was increased (n=4-6, 17 weeks old).

Fig.3 Detection of CD44 expression on CD3+T cells in MLN and spleen of WT (C57BL/6) mice and pCD2-hTL1A-Tg mice by FACS.

 In CD3+ T cells, compared with wild-type mice, the number of  CD44+ cells in the spleen and MLN of pCD2-hTL1A-Tg mice was increased (n=4-6, 17 weeks old).

Fig.4 Detection of CD86 expression on CD11c+DCs in MLN and spleen of WT (C57BL/6) mice and pCD2-hTL1A-Tg mice by FACS.

In DCs cells, the number of CD86+ cells in the spleen and MLN of pCD2-hTL1A-Tg mice was similar to that of wild-type mice (n=4-6, 17 weeks old).

Fig.5 Detection of CD44 expression on macrophages in MLN and spleen of WT (C57BL/6) mice and pCD2-hTL1A-Tg mice by FACS（n=4-6, 17 weeks old).

In macrophages, the number of CD86+ cells in the spleen and MLN of pCD2-hTL1A-Tg mice was similar to that of wild-type mice.

Fig.6 Detection of IFN-γ expression on CD4+T cells in MLN and spleen of WT (C57BL/6) mice and pCD2-hTL1A-Tg mice by FACS.

In CD4+ T cells, compared with wild-type mice, the number of IFNγ+ cells in the spleen and MLN of pCD2-hTL1A-Tg mice was significantly increased (n=4-6, 17 weeks old).

Fig.7 Detection of IL-17A+ expression on CD4+T cells in MLN and spleen of WT (C57BL/6) mice and pCD2-hTL1A-Tg mice by FACS.

In CD4+ T cells, compared with wild-type mice, the number of IL-17A+ cells in the spleen and MLN of pCD2-hTL1A-Tg mice was significantly increased (n=4-6, 17 weeks old).

Fig.8 Detection of IL-4+ expression on CD4+T cells in MLN and spleen of WT (C57BL/6) mice and pCD2-hTL1A-Tg mice by FACS.

No IL-4+ cells were observed in the spleen and MLN of pCD2-hTL1A-Tg mice and wild-type mice (n=4-6, 17 weeks old).

Fig. 8 Phenotype of WT (C57BL/6) mice and pCD2-hTL1A-Tg mice (n=2-3, 17 weeks old). (A) Gross photograph; (B) Colon photo; (C) Body weight and intestine index.

Fig. 9 H&E staining of the small intestine and colon in pCD2-hTL1A-Tg mice (n=2-3, 17 weeks old).