Prom1-CreERT2

品系全名

C57BL/6JSmo-Prom1em1(CreERT2-PolyA)Smoc

目录号

NM-KI-200169

品系状态

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基因信息

基因名
Prom1

品系描述

将CreERT2-PolyA插入到小鼠Prom1基因起始密码子处。 Prom1(prominin 1)是一种在干细胞表面表达的跨膜糖蛋白,Prom1-CreERT2与含有loxP序列的品系杂交时,诱导Cre 介导的重组会导致后代floxed侧翼序列的缺失,可用于追踪成体干细胞。
应用领域:Cre工具鼠,成体干细胞谱系等区域

验证数据

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图1 经他莫昔芬诱导后,tdtomato表达在Prom1-CreERT2; Rosa26-tdTomato小鼠的第三脑室。

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图2 经他莫昔芬诱导后,tdtomato表达在Prom1-CreERT2; Rosa26-tdTomato小鼠的支气管上皮。

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图3 经他莫昔芬诱导后,tdtomato大量表达在大脑,胰腺,肾,肺支气管,大肠,小肠,唾液腺,胃,胸腺(漏表达);在视网膜,卵巢,子宫有个别细胞表达。在肝有少量表达。对照组在视网膜、卵巢、肝有泄露表达。(想要获取更多信息需联系技术人员)



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