Nkx2-5-IRES-Cre

品系全名

C57BL/6JSmo-Nkx2-5em1(IRES-Cre-WPRE-polyA)Smoc

目录号

NM-KI-200152

品系状态

精子冻存

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基因信息

基因名
Nkx2-5

品系描述

将IRES-Cre-WPRE-polyA插入到小鼠Nkx2-5基因终止密码子处。
应用领域:Cre工具鼠,Nkx2.5是心脏发育的关键参与者。Nkx2-5-cre与报告小鼠交配的双阳性小鼠,可以标记Nkx2.5阳性细胞;与含flox的小鼠交配后,可以在心脏中剔除目的基因。

验证数据

image.png

图 1 Nkx2-5Cre/+; Rosa26tdTomato/+ 小鼠心脏中tdTomato的表达情况。

Nkx2-5Cre/+; Rosa26tdTomato/+双转基因鼠,可在小鼠心肌细胞和血管细胞中检测到tdTomato的表达。

image.png

图 2 Nkx2-5Cre/+; Rosa26tdTomato/+ 小鼠各组织中tdTomato的表达情况。TdTomato可以在心脏、肺支气管和肺泡、颌下腺中检测到。其他组织如脑、视网膜脉络丛、毛囊、肾、胰腺、小肠、大肠、脾脏和胸腺中少量个别细胞中表达tdTomato。不表达组织包括:垂体、肌肉。(更多详细信息请联系我司技术顾问。)




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