Foxj1-EGFP-CreERT2

品系全名

C57BL/6JSmo-Foxj1em1(GFP-CreERT2-polyA)Smoc

目录号

NM-KI-200133

品系状态

精子冻存

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基因信息

基因名
Foxj1

品系描述

将GFP-CreERT2-polyA插入到小鼠Foxj1基因起始密码子处。
应用领域:GFP-CreERT2 的表达模式与Foxj1基因表达的内源模式相似。他莫昔芬诱导的 cre 重组酶活性在肺、脑室的细胞层中检测到。可用于脑积水的研究。

验证数据

Foxj1-GFP-CreERT2-1.png

图 1  在Foxj1GFP-CreERT2/+; Rosa26tdTomato/+ 小鼠肺部,tdTomato和EGFP的表达情况。

经Tamoxifen诱导后,Foxj1GFP-CreERT2/+; Rosa26tdTomato/+ 双转基因鼠,可在小鼠肺支气管上皮细胞中表达tdTomato和GFP。Tamoxifen 诱导前有少数细胞漏表达。

Foxj1-GFP-CreERT2-2.png

图 2  在Foxj1CreERT2/+; Rosa26tdTomato/+ 小鼠脑部,tdTomato和EGFP的表达情况。

经Tamoxifen诱导后,Foxj1CreERT2/+; Rosa26tdTomato/+ 双转基因鼠,可在小鼠脉络丛细胞检测到tdTomato的表达,而检测不到EGFP的表达。Tamoxifen诱导前有少数细胞漏表达。

Foxj1-GFP-CreERT2-3.png

图 3 Foxj1CreERT2/+; Rosa26tdTomato/+ 小鼠各组织中tdTomato的表达情况。在12-13周雌性小鼠体内,未见表达的组织包括:胸腺、心脏、肝脏、胃、唾液腺、颌下腺、棕色脂肪,肾脏、胰腺、小肠和大肠、脾脏、卵巢和子宫、肌肉、皮肤。(更多详细信息请联系我司技术顾问。)


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