Notch1-CreERT2

品系全名

C57BL/6JSmo-Notch1em1(CreERT2-2xpA)Smoc

目录号

NM-KI-200122

品系状态

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基因信息

基因名
Notch1

品系描述

将CreERT2-2xpA插入到小鼠Notch1基因起始密码子处。 Notch1 可用于研究正常形态发生、分化、细胞增殖和细胞凋亡等多个过程。Notch1-CreERT2小鼠与含有loxP侧翼序列的小鼠交配,Cre介导的重组将导致后代表达Cre的细胞中floxed 序列的缺失。
应用领域:Cre工具鼠,反复活化的细胞如:内皮等区域

验证数据

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图1 经他莫昔芬诱导后,tdTomato表达在Notch1-CreERT2; Rosa26-tdTomato小鼠小肠的个别细胞中。

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图2 经他莫昔芬诱导后,tdtomato表达在Notch1-CreERT2; Rosa26-tdTomato小鼠肝脏的个别细胞中。

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图3 经他莫昔芬诱导后,tdtomato还会在脑、心脏、棕色脂肪、唾液腺、肺、肝、胃、肾脏、胰腺、表皮、大肠、脾脏、胸腺、肌肉的个别细胞中出现信号。(想要获取更多信息需联系技术人员)



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