Ptf1a-CreERT2

品系全名

C57BL/6JSmo-Ptf1aem1(CreERT2-WPRE-pA)Smoc

目录号

NM-KI-200118

品系状态

胚胎冻存

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基因信息

基因名
Ptf1a

品系描述

将CreERT2-WPRE-pA插入到小鼠Ptf1a基因起始密码子处。
应用领域:Cre工具鼠,胰腺多能祖细胞等区域

验证数据

Ptf1a-CreERT2-1.png

图 1  在Ptf1aCreERT2/+ ; Rosa26tdTomato/+ 小鼠胰腺中,tdTomato的表达情况。

经Tamoxifen诱导后,Ptf1aCreERT2/+ ; Rosa26tdTomato/+ 双转基因鼠,可在小鼠胰腺腺泡细胞中表达tdTomato。

Ptf1a-CreERT2-2.png

图 2  Tamoxifen 诱导后,Ptf1aCreERT2/+; Rosa26tdTomato/+ 小鼠各组织中tdTomato的表达情况。

CreERT2重组酶的活性特异表达在胰腺腺泡,有少量细胞漏表达;在小脑、垂体和胃粘膜下层有极少量细胞表达。未检测到表达的组织包括:胸腺、心脏、肺、肝、睾丸、肾、小肠、棕色脂肪、脾、颌下腺、肌肉。(更多详细信息请联系我司技术顾问。)


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