Gli1-CreERT2

品系全名

C57BL/6JSmo-Gli1em1(CreERT2-Wpre-polyA)Smoc

目录号

NM-KI-200073

品系状态

活体

导出PDF

基因信息

基因名
Gli1

基因曾用名

Zfp5; Zfp-5; AV235269

NCBI ID

14632

MGI ID

95727

Ensembl ID

ENSMUSG00000025407

Pubmed

Gli1

人类同源基因

GLI1

品系描述

将CreERT2-Wpre-polyA插入到小鼠Gli1基因起始密码子处。 Gli1是锌指蛋白Kruppel家族的成员。编码的转录因子由sonic hedgehog信号转导级联激活，并调节干细胞的增殖。该蛋白的活性和核定位受到p53的负向调节，形成一个抑制性循环。当这些Gli1-CreERT2小鼠与含有感兴趣的loxP侧翼序列的小鼠交配时，他莫昔芬诱导的Cre介导的重组将导致Gli1表达细胞中侧翼序列的缺失；使它们有助于研究胚胎发育的不同阶段刺猬反应细胞的轴型、增殖和细胞命运规范。
应用领域：Cre工具鼠; Hedgehog通路

验证数据

image.png

图1 经他莫昔芬诱导后，tdtomato表达在Gli1-CreERT2; Rosa26-tdTomato一月龄小鼠的颅面骨缝线的细胞中。(A)整体观察；（B）切片观察。


image.png

图2 经他莫昔芬诱导后，tdtomato表达在颅面骨缝线、皮层、海马和丘脑的细胞中，腺垂体、颌下腺、肾脏、卵巢，大肠、小肠、皮肤毛囊、肺泡、支气管的部分细胞中，以及在肝脏、心脏、肌肉、脾脏的极少量细胞中；而不表达在胰腺和胸腺中。(想要获取更多信息需联系技术人员)



发表文献 1篇

1. Targeting injury-triggered grancalcin from senescent macrophages enhances aged bone healing
发表年份：2025 来源杂志：Acta Biomaterialia

展开

你也可能感兴趣

Shanghai Model Organisms Center Inc has licensed CRISPR-Cas9 technology from Broad Institute

On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.

查看

Workshop：Progress and Advances in Preclinical immuno-Oncology Research

SMOC’s Annual Progress and Advances in Preclinical immuno-Oncology Research: The workshop is designed as a forum for ideas and opinions exchange on how to decrease the rate of clinical failures in oncology and immuno-oncology.

查看

Customizing Mouse Models: Delivering in 100 Days to Speed Up Your Research!

At GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.

查看